TABLE 3.

RFLPs of ITS regions for basidiomycetes

Species and isolate(s)a	RFLPs (bp) obtained with:
	AluI	HaeIII	TaqI	TaqI-HaeIII	Uncut ampliconb
Brown-rot basidiomycetes
Coniophora puteana	500	470	530	247
Fp-90099-Sp	187	242	185	197
	170	114	71	182
	91	59		69
		23		59
				41
				23
Total	948	908	786	813	970
Fomitopsis pinicola	380	545	310	248
K8Sp	200	127	203	165
	90	98	165	107c
	52	62	130	62
Total	722	832	870	796	930
Gloeophyllum trabeum	435	670	500	420
This lab	155	107	318	215
Mad-617-R	105	72	72	107
ATCC 11539	86			72
Total	781	849	890	814	890
Gloeophyllum sepiarum	435	670	500	420
10-BS2-2	155	107	170	140
	105	72	140	107
	86		72	72
Total	781	849	882	739	890
Leucogyrophana pinastri	580	320	400	245
This lab	300	245	180	127
	100	170	127	95
		140	95	72
		63	78	63
		55	63	37
Total	980	993	943	639	1,050
Postia placenta	448	610	530	445
This lab	240	110d	335	115d
Mad-698-R	100	72	72	72
Total	788	902	937	747	930
Serpula lacrimans	810	800	485	485
Harm-888-R	100	108	215	215
ATCC 36335			81	77
			68	68
			50	39
Total	910	908	899	884	930
White-rot basidiomycetes
Irpex lacteus
KTS 003	500	425	270	245
ATCC 60993	105	345	245	140
	93	105	150	108
		61	108	77
			77	61
			61
Total	698	936	911	631	960
Lentinula edodes	515	860	540	540
117=lt(d)	235	102	355	255
	105	50	60	102
				60
Phanerochaete chrysosporium	515	705	385	385
This lab	165	98	315	220
	94	50	98	98
			65	65
Total	774	853	863	768	850
Phanerochaete chrysosporium	515	705	330	330
ATCC 24725	165	98	300	208
	94	50	98	98
			59	59
			32	32
Total	774	853	819	727	850

Resinicium bicolor	380	800	270	270
HHB-8850-sp	150	50	245	245
ATCC 44175	98		215	215
ATCC 64897	69		60	60
Total	697	850	790	790	850
Scytinostroma galactinum	595	540	315	315
MB-1880-sp	204	268	200	137
	94	60	164	116
			75	75
			60	60
				50
Total	893	868	814	753	930
Scytinostroma galactinum	595	540	220	220
ATCC 44178	204	268	200	137
	94	60	116	116
			91	91
			75	75
			60	60
				50
Total	893	868	762	749	930
Scytinostroma galactinum	595	540	200	137
ATCC 64896	204	268	137	116
	94	60	116	91
			91	75
			75	60
			60	50
Total	893	868	679	529	930
Trametes versicolor	360	300d	320	260
This lab	146	105	172	135
	93	70	74	108
	74			74
	58			50
Total	731	775	566	627	910
Trametes versicolor	360	300d	320	255
Fp-101664-Sp	146	210	172	205
	93	70	74	135
	74			113
	58			74
				50
Total	731	880	566	832	910
Trichaptum abietinum	405	900	470	335
1247 MJL	365	310	335	145
	215	145	53	62
	155	53
	50
Total	1,190	1,408	858	542	1,460
Ectomycorrhiza
Pisolithus tinctorius	435	605	208	208
ATCC 38054	182	170	150	127
	165	59	127	100
	87		76	69
			59	59
			23	44
				23
Total	869	834	643	630	890
Soil-borne pathogen of herbaceous plants
Rhizoctonia solani	415	620	500	335
1AP	245	260	335	260
	98		59	242
	83			59
Total	841	880	894	896	970

^aAll isolates of a species which have identical RFLP profiles per restriction endonuclease for all enzymes are listed together. If different isolates of a species have different RFLP profiles for at least one of the restriction endonucleases, then those isolates are listed separately. 

^bAmplicon of the ITS region amplified by primers ITS1-F (specific for higher fungi) and ITS4-B (specific for basidiomycetes). 

^cThis fragment is a triplet, as reflected in the fragment total. 

^dThis fragment is a doublet, as reflected in the fragment total.