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. 2022 Jun 14;10:894810. doi: 10.3389/fcell.2022.894810

FIGURE 5.

FIGURE 5

METTL3 regulated the invasion of trophoblast by altering the stability and expression of ZBTB4. (A,B) HTR-8 cells were transfected with siCtrl, siMETTL3, vector or METTL3-expressing plasmid for 24 h; a time course for mRNA stability was initiated by adding an RNA-polymerase II inhibitor [actinomycin D (5 μg/ml)]. Cells were harvested at the indicated time points. Expression levels were normalized to that at “0 h,” and GAPDH mRNA was used as the reference gene. The results are shown as the mean of at least three independent experiments. *p < 0.05 versus the vector or siCtrl. (C) Results of immunofluorescence. Magnification = ×400. (D,E) METTL3 overexpression in HTR-8 cells increased trophoblast invasion compared to that of the control. The invasive ability of the cells was assessed using Image-Pro Plus 6.0 software. (Panel: original magnification ×100 magnification). (F,G) Images were taken over time at 0 and 24 h. White dashed lines delineate the wound area. Magnification = ×40.