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. 2022 Jun 14;12:873096. doi: 10.3389/fcimb.2022.873096

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Copyright © 2022 Tang, Yi, Lu, Chen and Liu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of BHD on the modified neurological severity score (mNSS), pathological changes and neuronal damage of rats with CI. (A) Animal experimental design. (B) Comparison of mNSS between different groups (n = 8 per group). (C) HE staining at a magnification of 20× (n = 4 per group). (D, E) Immunostaining and quantification of NeuN-positive cells in the hippocampal CA1 region (n = 4 per group). (F, G) Nissl staining and cell counts of Nissl bodies in the cortical area (n = 4 per group). All the data are presented as the mean ± SD. Differences were assessed by one-way ANOVA and Student’s t-test: ***p < 0.001, *p < 0.05.