Table 3.
Discriminant variables measured during the OGTT.
| A | B | t test | Pearson corr x Glucose | A/B | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean | SEM | N | Mean | SEM | N | p-Value | q value | rho | p-value | ||
| Bile acids | |||||||||||
| TUDCA (%) | 0.33 | 0.06 | 30 | 0.20 | 0.02 | 35 | 0.021 | 0.087 | 0.06 | 0.6505 | 1.67 |
| Unconj. BA (%) | 38.59 | 2.83 | 33 | 31.36 | 2.67 | 39 | 0.068 | 0.132 | 0.24 | 0.0414 | 1.23 |
| Conj./ Unconj. BA | 2.95 | 0.41 | 33 | 4.44 | 0.53 | 39 | 0.034 | 0.088 | −0.31 | 0.0079 | 0.66 |
| Gly-conj. BA (%) | 51.85 | 2.26 | 33 | 60.36 | 2.28 | 39 | 0.011 | 0.068 | −0.28 | 0.0179 | 0.86 |
| Gly-conj. BA (nmol/L) | 1,597.61 | 127.71 | 33 | 2,345.40 | 273.06 | 39 | 0.022 | 0.087 | −0.26 | 0.0262 | 0.68 |
| GCDCA (nmol/L) | 853.72 | 79.15 | 33 | 1,164.85 | 106.33 | 39 | 0.026 | 0.087 | −0.25 | 0.0380 | 0.73 |
| Fatty acid-derived acylcarnitines | |||||||||||
| C14 (μmol/L) | 0.02 | 0.00 | 33 | 0.02 | 0.00 | 39 | 0.028 | 0.087 | 0.35 | 0.0028 | 1.18 |
| C16 (μmol/L) | 0.11 | 0.01 | 33 | 0.10 | 0.00 | 39 | 0.033 | 0.088 | 0.39 | 0.0007 | 1.14 |
| C18 (μmol/L) | 0.03 | 0.00 | 33 | 0.02 | 0.00 | 39 | 0.039 | 0.096 | 0.31 | 0.0083 | 1.16 |
| C3 (μmol/L) | 0.34 | 0.02 | 33 | 0.26 | 0.01 | 39 | 0.004 | 0.055 | 0.47 | 3.13E-05 | 1.28 |
| C4 (μmol/L) | 0.07 | 0.01 | 33 | 0.05 | 0.00 | 39 | 0.018 | 0.087 | 0.37 | 0.0014 | 1.37 |
| C6 (μmol/L) | 0.04 | 0.00 | 33 | 0.03 | 0.00 | 39 | 0.018 | 0.087 | 0.39 | 0.0008 | 1.34 |
| Amino acids and amino acid-related metabolites | |||||||||||
| Isovaleryl-carnitine (μmol/L) | 0.08 | 0.01 | 33 | 0.06 | 0.00 | 39 | 0.011 | 0.068 | 0.56 | 4.00E-07 | 1.25 |
| Spermidine (μmol/L) | 0.18 | 0.03 | 33 | 0.12 | 0.01 | 39 | 0.041 | 0.097 | 0.41 | 0.0004 | 1.53 |
| Spermine (μmol/L) | 0.25 | 0.06 | 33 | 0.13 | 0.01 | 39 | 0.031 | 0.087 | 0.33 | 0.0052 | 1.90 |
| Urea (mmol/L) | 5.94 | 0.21 | 33 | 5.34 | 0.20 | 39 | 0.047 | 0.100 | 0.13 | 0.2626 | 1.11 |
| Free carnitine (μmol/L) | 39.65 | 1.49 | 33 | 35.94 | 1.30 | 39 | 0.064 | 0.129 | 0.26 | 0.0290 | 1.10 |
| Glu (μmol/L) | 51.52 | 4.43 | 33 | 31.41 | 2.39 | 39 | 0.0001 | 0.0017 | 0.64 | 1.39E-09 | 1.64 |
| Ile (μmol/L) | 59.61 | 2.20 | 33 | 53.16 | 1.90 | 39 | 0.029 | 0.087 | 0.56 | 3.31E-07 | 1.12 |
| Leu (μmol/L) | 114.85 | 4.51 | 33 | 101.89 | 3.12 | 39 | 0.018 | 0.087 | 0.52 | 2.21E-06 | 1.13 |
| Phe (μmol/L) | 53.84 | 1.38 | 33 | 49.93 | 1.09 | 39 | 0.028 | 0.087 | 0.30 | 0.0114 | 1.08 |
| Trp (μmol/L) | 54.19 | 1.26 | 33 | 49.66 | 1.20 | 39 | 0.011 | 0.068 | 0.33 | 0.0051 | 1.09 |
| Val (μmol/L) | 202.08 | 6.18 | 33 | 186.47 | 4.78 | 39 | 0.046 | 0.100 | 0.53 | 1.30E-06 | 1.08 |
| Gln (μmol/L) | 575.08 | 11.52 | 33 | 622.93 | 12.04 | 39 | 0.006 | 0.056 | −0.29 | 0.0140 | 0.92 |
| Gly (μmol/L) | 206.06 | 9.64 | 33 | 247.27 | 12.05 | 39 | 0.011 | 0.068 | −0.31 | 0.0077 | 0.83 |
| Ser (μmol/L) | 92.49 | 2.74 | 33 | 101.72 | 3.18 | 39 | 0.035 | 0.088 | −0.22 | 0.0602 | 0.91 |
| Glucose metabolism | |||||||||||
| Glucose (mmol/L) | 7.62 | 0.21 | 33 | 5.64 | 0.09 | 39 | <0.000001 | <0.000001 | 1 | 1.35 | |
| Insulin (mIU/L) | 80.99 | 5.94 | 33 | 49.65 | 4.13 | 39 | 0.00003 | 0.00130 | 0.56 | 2.87E-07 | 1.63 |
| Mannose (AU) | 0.82 | 0.05 | 33 | 0.69 | 0.05 | 39 | 0.056 | 0.116 | 0.47 | 2.95E-05 | 1.18 |
| Meso-Erythritol (AU) | 1.42 | 0.10 | 33 | 1.18 | 0.05 | 39 | 0.030 | 0.087 | 0.24 | 0.0413 | 1.20 |
| Threitol (AU) | 1.41 | 0.10 | 33 | 1.17 | 0.04 | 39 | 0.023 | 0.087 | 0.27 | 0.0225 | 1.21 |
| Pyruvate (AU) | 1.22 | 0.10 | 33 | 0.98 | 0.07 | 39 | 0.042 | 0.097 | 0.37 | 0.0013 | 1.25 |
| Lipids | |||||||||||
| Palmitic acid (AU) | 2.35 | 0.12 | 33 | 2.01 | 0.09 | 39 | 0.029 | 0.087 | 0.31 | 0.0080 | 1.17 |
| Stearic acid (AU) | 2.13 | 0.09 | 33 | 1.81 | 0.07 | 39 | 0.005 | 0.055 | 0.32 | 0.0060 | 1.18 |
| Cholesterol (AU) | 2.57 | 0.19 | 33 | 2.09 | 0.14 | 37 | 0.045 | 0.100 | 0.16 | 0.1951 | 1.23 |
| Triglycerides (mmol/L) | 1.52 | 0.08 | 33 | 1.09 | 0.06 | 39 | 0.0001 | 0.0017 | 0.52 | 2.37E-06 | 1.39 |
| Leucocytes | |||||||||||
| Monocytes (giga/L) | 0.50 | 0.03 | 33 | 0.39 | 0.02 | 39 | 0.005 | 0.055 | 0.25 | 0.0340 | 1.27 |
| Neutrophils (giga/L) | 3.88 | 0.46 | 33 | 2.83 | 0.14 | 39 | 0.023 | 0.087 | 0.23 | 0.0518 | 1.37 |
| White blood cells (giga/L) | 6.31 | 0.49 | 33 | 4.98 | 0.18 | 39 | 0.008 | 0.068 | 0.27 | 0.0206 | 1.27 |
The variables measured in samples collected during the OGTT that received a VIP value > 1 in the PLS-DA model at a minimum of two time points are presented with the average concentration during the test. The differences between the 2 clusters were tested with a T-test and their correlation (Pearson) with the average glucose concentration during the OGTT is presented. The right (last) column presents the ratio of each variable between cluster A and B individuals. Highlighted rows indicate variables predominantly associated with cluster B. Variables not considered different between the two clusters after the T-test were not included in the table.