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Validation of RNA-seq results using quantitative reverse-transcription PCR (qRT-PCR). Expression profiles of nine DEGs playing important roles in viral defense. qRT-PCR verified that mRNA levels of these genes were increased after viral infection, consistent with the altered expression profiles observed in transcriptome data. β-actin was used for normalization. The x-axis represents the different time points, and the y-axis represents the normalized fold-change of transcripts.
