In the original article, there was a mistake in Supplementary Figure S1 , as published. Supplementary Figure S1B was mistakenly replaced by Figure S2 , which appears thus duplicated, and the word “Hoetschst” was mispelled, the correct spelling of this word being “Hoechst”. The corrected Supplementary Figure S1B is shown below.
In addition, there was a mistake in Figure 1 and Supplementary S2 , as published. The word “Hoetscht” was misspelled. The correct spelling of this word is “Hoechst”. The corrected Figure 1 and Supplementary S2 are shown below.
Figure 1.
Optimization of hepatocyte culture systems for modeling persistent Listeria infection. Different cell seeding conditions, MOI and Listeria strains (EGDe or 10403S) were tested to obtain optimal long-term Listeria infection of HepG2 (see Supplementary Figure S1 ), Huh7 or PMH. Infected cells were examined at day 1 (d1) and at day 3 (d3) by immunofluorescence microscopy: representative examples under optimized conditions are shown. (A) Low magnification micrographs of Huh7 cells infected with EGDe strain (MOI=1-5) or PMH infected with 10403S strain (MOI=10) for the indicated time. Images are overlays of Listeria (green) and Hoechst (blue) signals (bars: 50 μm, Huh7, or 200 μm, PMH). (B) High magnification micrographs of infected Huh7 or PMH showing Listeria (green), F-actin (red) and Hoechst (blue) signals. Bars: 5 μm. Boxed regions enlarged on the right show F-actin (top) or merged signals (bottom), highlighting actin-positive bacteria at d1 and actin-negative bacteria at d3 (bars: 1 μm). (C) Micrographs of an infected Huh7 cell at d3, showing Listeria (green), LAMP1 (red) and Hoechst (blue) signals. Arrows indicate 3 examples of LisCVs. (D) Phase contrast image of an infected PMH at d3 (bars: 10 μm). Arrows indicate 2 examples of bacteria within vacuoles, shown at a higher magnification on the right (bars: 1 μm).
The authors apologize for these errors and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
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Supplementary Material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2022.911320/full#supplementary-material
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