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. 2022 Mar 28;189(3):1314–1325. doi: 10.1093/plphys/kiac148

Figure 4.

Figure 4

The activation of the ROS wave following wounding is suppressed in mutants deficient in the eATP receptors P2Ks. A, Representative time-lapse images of whole plant ROS levels (indicated by DCF oxidation) in WT (Col-0) and p2k1-3, p2k2, and p2k1-3p2k2 plants following wounding of a single leaf (local; L). B, Statistical analysis of L and systemic (S) ROS levels in leaves of wounded or unwounded (on leaf L) WT and p2k1-3, p2k2, and p2k1-3p2k2 mutant plants at 20 min following wounding. All experiments were repeated at least three times with at least three treated and three untreated plants per repeat. Letters indicate statistical significance (P < 0.05) by one-way ANOVA (n = 6). Bar = 1 cm. Each box plot consists of borders corresponding to the 25th and 75th percentiles of the data, with data values included as points within the plot. The median is described by an inner, horizontal line and “X” corresponds to the mean. Whiskers describe the 1.5× interquartile range of the data.