Figure 2.

R induces changes in the level of APY protein in primary roots. R induces a decrease in APY protein expression in primary roots at early time points (A), and an increase 24 h after R exposure (B). After seedlings were grown for 2.5 days in darkness, they were irradiated with continuous R for 6, and 12 h (A), or maintained in darkness or irradiated with continuous R for 24 h (B). APY1 and APY2 levels were detected by anti-APY antibody, and the same blot was stripped and re-probed with anti-HIS3 antibody for equal loading control. Densitometry measurements of APY and HIS3 levels were measured in cotyledons (A), or in roots (B) by ImageJ.