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. 2022 Mar 31;189(3):1728–1740. doi: 10.1093/plphys/kiac150

Figure 4.

Figure 4

R induces an increase in the level of APY protein in apical hook-cotyledon tissue. The increase in the level of APY protein induced by R in apical hook-cotyledons is gradual, rising to consistently 1.3-fold or higher at and after 6 h of R irradiation. After Ws WT seedlings were grown for 2.5 days in darkness, they were irradiated with continuous R for 2, 4, 6,12, 24, and 72 h. APY1 and APY2 levels were detected by anti-APY antibody, and the same blot was stripped and re-probed with anti-HIS3 antibody for equal loading control. Densitometry measurements of APY and HIS3 levels were measured by ImageJ.