Figure 5.

On media without sucrose added, R does not induce a significant increase in APY protein levels if seedling photosynthesis is inhibited by DCMU, and R significantly promotes root growth A, Ws WT seedlings grown in dark, or transferred to continuous R after 2.5 days growth in dark, were grown under R with no treatment, with DMSO (0.001%V/V) or with 50 μM DCMU dissolved in DMSO (0.001% V/V) for 72 h. APYl and APY2 levels were detected by anti-APY antibody, and the same blot was stripped and reprobed with anti-HIS3 antibody for equal loading control. Densitometry measurements of APY and HIS3 levels were measured by ImageJ, and the signal ratio is expressed relative to the dark sample. B, The length of roots in both R-treated and dark-grown apy1 and apy2 seedlings is significantly less than that observed in Ws WT seedlings. Shown is a representative result that was observed in samples independently grown on three separate plates, each of which had an n value of 10. The R treatment in both (A) and (B) was for 24 h. Different letters above the bars indicate values that differ significantly from each other (P < 0.05, Student’s t test; error bars are se).