Figure 2.

Overexpression of Re shows a red foliated phenotype but not in the fiber. A, Tissue expression pattern of Re between E22 and ReS_11.Re was highly expressed in the main tissues of ReS₁₁, except in the fiber. DPA is the abbreviation of days post-anthesis. B, Tissue expression patterns of overexpression lines CK1 and OE1. Re was highly expressed in the main tissues of OE1, except for in the fiber. For (A) and (B), error bars represent the ±sd (three biological replicates), and the statistical significance was calculated using a t test (**P < 0.01, *P < 0.05). C, Anthocyanins accumulated at different stages during genetic transformation. From left to right are the calli, seedling regeneration, rooting culture and transgenic T0 plants from the transformation of 35S:Re. Normal light green and obvious red calli were observed from the callus stage. The regenerated seedlings showed red or green color (transgenic negative control). Finally, transgenic plants with different shades of red were obtained. D, Neither OE1 nor ReS₁₁ produced a red color in the developing or the mature fibers.