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. Author manuscript; available in PMC: 2023 Jul 1.

Published in final edited form as: J Magn Reson. 2022 May 13;340:107234. doi: 10.1016/j.jmr.2022.107234

Fig. 8. — Schematic representation of CD22-CAR structure as a function of the CD8α and CD28 hinge domains. (A) Diagram of the CAR construct used in this study harboring the CD22-targeting m971 scFv (blue), CD8α TM (brown), 4–1BB costimulatory domain (yellow), as well as TCR ζ (red) as indicated. Four model structures (black, blue, green or orange) generated by Schrödinger maestro represent the CD8α H in the different states observed in NMR spectra. A distance of 15 nm is indicated as representing the intermembrane distance for T cell interaction with an antigen-presenting cell. (B) Amino acid sequence of the CD28 H and CD8α H under study with the prolines highlighted in red.

Fig. 8. — Schematic representation of CD22-CAR structure as a function of the CD8α and CD28 hinge domains. (A) Diagram of the CAR construct used in this study harboring the CD22-targeting m971 scFv (blue), CD8α TM (brown), 4–1BB costimulatory domain (yellow), as well as TCR ζ (red) as indicated. Four model structures (black, blue, green or orange) generated by Schrödinger maestro represent the CD8α H in the different states observed in NMR spectra. A distance of 15 nm is indicated as representing the intermembrane distance for T cell interaction with an antigen-presenting cell. (B) Amino acid sequence of the CD28 H and CD8α H under study with the prolines highlighted in red.