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. Author manuscript; available in PMC: 2023 Jun 9.
Published in final edited form as: Cell. 2022 May 12;185(12):2071–2085.e12. doi: 10.1016/j.cell.2022.04.025

Figure 6. Antibody mediated depletion of inflammatory cell lineages in mice and RNAseq reveal macrophage recruitment by SADBE.

Figure 6.

(A) Quantification of MelanA positive cells per mm2 in Dct-Cre NrasQ61R mice after immunodepletion by IP injection with 750 μg of anti F4/80, anti-CD-4, anti-NK1.1, anti-CD-8 and anti-CD-19. (B) Quantification of INOS positive cells per mm2 in Dct-Cre NrasQ61R mice after immunodepletion by IP injection with 750 μg of anti F4/80 and anti-CD-4. (C) Arg1 staining (red) of ear skin sections following depletion with anti-CD-4, anti-F4/80, isotype control and 2% SADBE treatment for 1 week. Yellow arrows indicate negative signals. Melanin detected by bright-field microscopy is shown in white. (D) Differential gene expression analysis (E) GSEA hallmark pathways analysis (F) Heat map of macrophage marker groups according to macrophage phase and (G) GO biological processes analysis. Dct-Cre NrasQ61R mice were treated with SADBE (2%) or vehicle control (acetone) on dorsal skin (3 mice per treatment or control group). Whole skin RNA-Seq was performed 72h after treatment. Macrophage-associated genes for M0, M1, and M2 phases were collected from the hematopoietic gene signature set LM22.