Table 1.

Primers and probes

Organism	Primer	Sequence	Target	References
Tick-borne encephalitis virus	F-TBE1	5′-GGGCGGTTCTTGTTCTCC-3′	Primers, 5′ untranslated region	[28]
	R-TBE1	5′-ACACATCACCTCCTTGTCAGACT-3′
	TBE-WT	5′-FAM-TGAGCCACCATCACCCAGACACA-RTQ1-3′	Probe, 5′ untranslated region
Borrelia sp.	BspF-16s	5′-GCTGTAAACGATGCACACTTGGT-3′	Primers, 16S rRNA gene of B. sp.	[29, 30]
	BspR-16s	5′-GGCGGCACACTTAACACGTTAG-3′
	FAM-LD	5′-FAM-TTCGGTACTAACTTTTAGTTAA-MGB-3′	Probe, 16S rRNA gene of B. burgdorferi s.l
	VIC-RF	5′-VIC-CGGTACTAACCTTTCGATTA-MGB-3′	Probe, 16S rRNA gene of B. miyamotoi
	Bfla-PAD	5′-GATCARGCWCAAYATAACCAWATGCA-3’	Primers, flagellin B gene of Borrelia sp.	[31]
	Bfla-PDU	5′-AGATTCAAGTCTGTTTTGGAAAGC-3’

rRNA Ribosomal RNA