Fig. 7.

Following NLRP3 knockout, pyroptosis is inhibited in mice infected with A. fumigatus after irradiation. a–c Representative western blot images showing changes in the levels of caspase 1-p20, and gasdermin D (GSDMD-N) in the lungs of wild-type (WT) and NLRP3^−/− mice and their gray value statistics. d-e The co-immunoprecipitation (CO-IP) results of ASC-NLRP3-inflammation and its gray value statistics. f Representative immunohistochemistry double-staining images of ASC and NLRP3 in the lung sections of WT and NLRP3^−/− mice. Red and green fluorescence indicate ASC and NLRP3, respectively. Scale bars: 100 μm. g–i Levels of lactate dehydrogenase (LDH), interleukin 18 (IL-18), and IL-1β in the bronchoalveolar lavage fluid (BALF) of WT and NLRP3^−/− mice in Af and RA + Af groups. Data are shown as the mean ± standard deviation (n = 5). *P < 0.05, **P < 0.01