TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference/source |
|---|---|---|
| Source plasmids | ||
| pSRKKm-Plac-sfgfp | pSRKKm vector containing lacIq and lac promoter with sfGFP | (90) |
| pNTPS139 | Kmr; suicide vector containing oriT and sacB | D. Alley |
| Deletion plasmids | ||
| pNTPS139 Δatu3077 | Kmr Sucs; deletion plasmid for atu3077 (ampC) | This study |
| pNTPS139 Δatu3078 | Kmr Sucs; deletion plasmid for atu3078 (ampR) | This study |
| pNTPS139 Δatu0009 | Kmr Sucs; deletion plasmid for atu0009 (mltA) | This study |
| pNTPS139 Δatu0092 | Kmr Sucs; deletion plasmid for atu0092 (mltB1) | This study |
| pNTPS139 Δatu2122 | Kmr Sucs; deletion plasmid for atu2122 (mltB2) | This study |
| pNTPS139 Δatu3779 | Kmr Sucs; deletion plasmid for atu3779 (mltB3) | This study |
| pNTPS139 Δatu1022 | Kmr Sucs; deletion plasmid for atu1022 (slt1) | This study |
| pNTPS139 Δatu2122 | Kmr Sucs; deletion plasmid for atu2112 (slt2) | This study |
| pNTPS139 Δatu2117 | Kmr Sucs; deletion plasmid for atu2117 (slt3) | This study |
| pNTPS139 Δatu3093 | Kmr Sucs; deletion plasmid for atu3093 (slt4) | This study |
| pNTPS139 Δatu2113 | Kmr Sucs; deletion plasmid for atu2113 (ampD) | This study |
| Replicating plasmids | ||
| Plac-atu3077 | Kmr; pSRKKm vector containing lacIq and lac promoter for the production of AmpC | This study |
| Plac-atu3078 | Kmr; pSRKKm vector containing lacIq and lac promoter for the production of AmpR | This study |
| Plac-atu2113 | Kmr; pSRKKm vector containing lacIq and lac promoter for the production of AmpD | This study |
| Plac-atu3779 | Kmr; pSRKKm vector containing lacIq and lac promoter for the production of MltB3 | This study |
| Strains | ||
| E. coli | ||
| DH5α | Cloning strain | Life Technologies |
| S17.1 | Smr; RP4-2, Tc::Mu,Km-Tn7, for plasmid mobilization | (91) |
| A. tumefaciens | ||
| C58 | Nopaline type strain; pTiC58; pAtC58 | |
| GV3101 | C58-derived; pTiC58DT-DNA; strain for Agrobacterium-mediated transformation of dicots | John Walker lab |
| FC2452 | Deletion strain for ΔmltA (atu0009) in C58 | This study |
| FC2444 | Deletion strain for ΔmltB1 (atu0092) in C58 | This study |
| FC2465 | Deletion strain for ΔmltB2 (atu2122) in C58 | This study |
| FC2487 | Deletion strain for ΔmltB3 (atu3779) in C58 | This study |
| FC2446 | Deletion strain for Δslt1 (atu1022) in C58 | This study |
| FC2448 | Deletion strain for Δslt2 (atu2112) in C58 | This study |
| FC2450 | Deletion strain for Δslt3 (atu2117) in C58 | This study |
| C58 ΔtetRA::a-attTn7 | Replacement of the ΔtetRA locus with an artificial attTn7 site | (90) |
| C58 ΔtetRA::a-attTn7 Δatu3077 | Deletion strain for ΔampC | This study |
| C58 ΔtetRA::a-attTn7 Δatu3077 pSRKKm-Plac-atu3077 | Kmr; deletion of ampC in C58ΔtetRA::a-attTn7 carrying complementing plasmid | This study |
| C58 ΔtetRA::a-attTn7 Δatu3078 | Deletion strain for ΔampR | This study |
| C58 ΔtetRA::a-attTn7 Δatu3078 pSRKKm-Plac-atu3078 | Kmr; deletion of ampR in C58ΔtetRA::a-attTn7 carrying complementing plasmid | This study |
| C58 ΔtetRA::a-attTn7 ΔampD | Deletion strain for ΔampD | This study |
| C58 ΔtetRA::a-attTn7 ΔampD pSRKKM-Plac-atu2113 | Kmr; deletion of ampD in C58ΔtetRA::a-attTn7 carrying complementing plasmid | This study |
| C58 ΔtetRA::a-attTn7 ΔampD ΔampC | Deletion strain for ΔampD ΔampC | This study |
| C58 ΔtetRA::a-attTn7 ΔampD ΔampR | Deletion strain for ΔampD ΔampR | This study |
| C58 ΔtetRA::a-attTn7 Δatu3093 | Deletion strain for Δslt4 | This study |
| C58 GV3101 ΔmltB3 | Deletion strain for ΔmltB3 | This study |