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. 2022 May 31;88(12):e00333-22. doi: 10.1128/aem.00333-22

TABLE 2.

Synthesized DNA primers used in this study

Synthesized DNAa Sequence
atu3077 P1 5′-GCGGCGACTAGTAAACGGATGCCGCTTTTGAAATGC-3′
atu3077 P2 5′-AAGCTTGGTACCGAATTCGCGATTAAATTTCATCTTTCGTGT-3′
atu3077 P3 5′-GAATTCGGTACCAAGCTTGCGCTCGAAAAGGCGCAATAA-3′
atu3077 P4 5′-GTCGTCGGATCCAGATAACTCGGCACACGCCCA-3′
atu3077 P5 5′-CTGCGCCGCCGGTGAAACGCCCGC-3′
atu3077 P6 5′-TGTGCCGGAGGCGCTTGCGATCGC-3′
atu3078 P1 5′-GTCGTCACTAGTGGGTTTTCCCTTCATGGACGG-3′
atu3078 P2 5′-AAGCTTGGTACCGAATTCAAATTGCCGAACCATTCAAGACCT-3′
atu3078 P3 5′-GAATTCGGTACCAAGCTTGAGACCATCGGAACGGCGTGA-3′
atu3078 P4 5′-GTCGTCGGATCCCCTGTTTGATGCTTTTTATCGCGC-3′
atu3078 P5 5′-CCAAGCGCCGGGAAAAGCGTGTCC-3′
atu3078 P6 5′-GGCATCGTCTGCGTGGTGTTCGTC-3′
atu2113 P1 5′-GTCGTCACTAGTCAGATTTTCGATTTCCCGGACGAA-3′
atu2113 P2 5′-AAGCTTGGTACCGAATTCCGAACATTCTTTCATGCGACG-3′
atu2113 P3 5′-GAATTCGGTACCAAGCTTCTGCCGAGATTTTCGGCCGCCTGA-3′
atu2113 P4 5′-GTCGTCGGTACCCCACCGAAACCACGGCATGCGCCA-3′
atu2113 P5 5′-GCGCAATCGGCGACGCGG-3′
atu2113 P6 5′-GTCGTCTGCTGCACACTTCGCCGC-3′
atu0009 P1 5′-AAAAAGCTTAACGCATCTTCTAGCCTTGCG-3′
atu0009 P2 5′-TATTCATTGCTCGGATTCGG-3′
atu0009 P3 5′-GCAATGAATATATCGGCGATGAAAGGC-3′
atu0009 P4 5′-AAAGGATCCGAAAGAACAATTCCTCCGC -3′
atu0009 P5 5′-TTTCGGCGACCTATGACAAGGACGG-3′
atu0009 P6 5′-TTACCAGTTTGCGGAACGCTGGG-3′
atu0092 P1 5′-AAACTGCAGATTCTTGCCCTGATGCCCATTGTCGC-3′
atu0092 P2 5′-AGACCGAATATCGTCTTTTAATGCTGGTCGG-3′
atu0092 P3 5′-ATTCGGTCTCCTCTTGGATGG-3′
atu0092 P4 5′-AAAGGATCCAAGTCGAGATCGACTGAGCCC-3′
atu0092 P5 5′-AATATGTCCGCCACAACCATCGTCGC-3′
atu0092 P6 5′-ATTACATCACAGACCGCCTCTCCG-3′
atu2122 P1 5′-AAAGAATTCAATCATCAGGGTTCCAATGCGG-3′
atu2122 P2 5′-TAGTGCGATTTTCCTCGATAGGTTGTTGGC-3′
atu2122 P3 5′-ATCGCACTACCGGGCCTTTAATCTATCGG-3′
atu2122 P4 5′-AAAAAGCTTATAATGACGTCTTTGAACGC-3′
atu2122 P5 5′-TATACCGCAACCGGCGTCGTACCCG-3′
atu2122 P6 5′-TTTCTGTGATGCGGTGCAGCACGG-3′
atu3779 P1 5′-AAACTGCAGTAGAAATTCGACGGCGCCG-3′
atu3779 P2 5′-TTTCGATTGCGAAAACGCATCGGGCG-3′
atu3779 P3 5′-GCAATCGAAATAATGTGCCGGCGAATTCGG-3′
atu3779 P4 5′-AAAGGATCCTTGGCCGTTCATGTCGTAGCC-3′
atu3779 P5 5′-TTTCGGAACTGCCTTGGTGGCGG-3′
atu3779 P6 5′-ATTCCCGGCCGGAACTACCATCGC-3′
atu2112 P1 5′- AAAAAGCTTTATTTCGTCTTCGAGGATGGG-3′
atu2112 P2 5′- GATGATCGATATGACAGAAACAGTGAAATGGC-3′
atu2112 P3 5′-ATCGATCATCTGCGAAATTGCG-3′
atu2112 P4 5′-AAAGAATTCATATCGTCCTCGGTTTCCGC-3′
atu2112 P5 5′- AAAAAGCTTTATTTCGTCTTCGAGGATGGG-3′
atu2112 P6 5′- TTTGCACCGAAAGATGCCGCG-3′
atu1022 P1 5′-AAAAAGCTTTTATGCGCTTTGACCAGCGCACCC-3′
atu1022 P2 5′-AATCCCCAGACTGTCTTTTTCATGCCG-3′
atu1022 P3 5′-TGGGGATTATTCAGGCACGGGCTAGCC-3′
atu1022 P4 5′-AAAGAATTCAATACGCTCTTCAACTCCATCCG-3′
atu1022 P5 5′- AAAAGCGACGTCGCTCGCG-3′
atu1022 P6 5′-AAACTACTACGACGAAGACGGTCAGG-3′
atu2117 P1 5′-AAAGAATTCAAGAGAATGTCTGGACAGGCGTGGC-3′
atu2117 P2 5′-ATTTTCAATCTAGAGTTGCCGCCTTCGTTATGCC-3′
atu2117 P3 5′- TTGAAAATGCTGCGGCTCCC-3′
atu2117 P4 5′-AAAAAGCTTTAGATGTTCCTGTCGAACACCG-3′
atu2117 P5 5′-ATACAGGCGCATCGCGGCC-3′
atu2117 P6 5′-AAGGCGAGGTGGTCACTGACC -3′
atu3093 P1 5′-GCTGCAACTAGTGCGCCACAGCCCATCTCG-3′
atu3093 P2 5′-AAGCATGGTACCGAATTCGCGGCTTGTTGATATTCCT-3′
atu3093 P3 5′-GAATTCGGTACCATGCTTTAGCAGCGGCGGGCGCCGGA-3′
atu3093 P4 5′-GCACGTAAGCTTGCGGTGATCTTGATGAT-3′
atu3093 P5 5′-GAGCTTCTCGGGCAATTCCG-3′
atu3093 P6 5′-GCCTCACGAAGCCGCACGATC-3′
atu3077 NdeI Fwd 5′- GCGGCGCATATGAAATTTAATCGCAGACAT-3′
atu3077 NdeI BamHI Rvs 5′- GTCGTCGGATCCTTATTGCGCCTTTTCGAG-3′
atu3078 NdeI Fwd 5′- GCGGCGCATATGGTTCGGCAATTTCTTCCC-3′
atu3078 BamHI Rvs 5′- GTCGTCGGATCCTCACGCCGTTCCGATGGT-3′
atu3779 Ndel Fwd 5′- GCGGCGCATATGACAAAGACCCTTTCAAAT-3′
atu3779 KpnI Rvs 5′- GCGGCGCATATGACAAAGACCCTTTCAAAT-3′
atu2113 NdeI Fwd 5′- GCGGCGCATATGAAAGAATGTCTGCCGGAT-3′
atu2113 BamHI Rvs 5′- GTCGTCGGATCCTCAGGCGGCCGAAAATCT-3′
a

Fwd, forward; Rvs, reverse.