FIG 3.

Determining the stability of SARS-CoV-2 proteins. Human lung epithelial BEAS-2B cells were exposed to cycloheximide (CHX) (100 μg/mL) at four time points (2, 4, 6, and 8 h) prior to immunoblotting. The bands were quantitated and normalized to β-actin, and the half-lives of the indicated proteins are shown graphically. (A) NSP1, E, and ORF8. (B) NSP3d, NSP4, NSP6, NSP7, NSP8, NSP9, NSP12, NSP13, NSP14, NSP16, ORF3a, ORF3b, ORF6, ORF7b, and ORF9b. (C) NSP2, NSP5, NSP10, NSP15, spike, M, and N. Data are from three independent biological replicates.