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. 2022 Jan 11;11(2):e021726. doi: 10.1161/JAHA.121.021726

Figure 4. TLR‐9–induced NF‐κB‐mediated inflammation in response to maternal plasma from women with preeclampsia and gestational age–matched healthy controls.

Figure 4

Nuclear factor kappa‐light‐chain‐enhancer of activated B cells (NF‐κB)‐mediated production of secreted embryonic alkaline phosphatase (SEAP) in (A) human embryonic kidney (HEK) 293 cells expressing an inducible SEAP reporter gene under the control of an NF‐κB inducible promoter (HEK‐Blue Null1, control cells; controls, n=16; preeclampsia, n‐15), (B) HEK293 cells overexpressing the human TLR‐9 gene and expressing an inducible SEAP reporter gene under the control of an NF‐κB inducible promoter (HEK‐Blue hTLR‐9; controls, n=18; preeclampsia, n=15), and (C) HEK‐Blue hTLR‐9 treated with chloroquine (CQ; controls, n=13; preeclampsia, n=15). All cells were exposed to 10% maternal plasma from women with preeclampsia or gestational age‐matched healthy pregnant women. Student’s t‐test. All values presented as median (white line), interquartile range (black lines). OD 630 nm, optical density at 630 nanometer wavelength light. Open squares=gestational age–matched control; open circles=third‐trimester pregnancies with preeclampsia.