Sequence alignment of C. botulinum plasmid pST7B and related plasmids using the Mauve analysis tool. Further information relative to the degree of conservation of pST7B with homologous plasmids/contigs is shown in Table S1. Colored regions correspond to homologous regions between the different plasmid sequences. Blue arrow, gene coding for a restriction-modification system; purple arrow, gene coding for a putative replication protein; green arrow, gene coding for DNA recombination protein; yellow arrow, gene coding for amidase/N-acetylmuramoyl-l-alanine-amidase; orange arrow, gene coding for a putative RNA polymerase sigma factor; gray arrow, genes for coding hypothetical proteins.