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. 2022 May 2;13(3):e02384-21. doi: 10.1128/mbio.02384-21

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Copyright © 2022 Douillard et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 3 — SNP analysis of all environmental (green) and stool isolates (red) of Clostridium botulinum. (a) Distribution of the isolates based on their SNP/InDel count. μ, median. (b) Neighbor-joining tree of all isolates based on Hamming distance (Saitou-Nei criterion) using PHYLOViZ software (64). (c) Chromosomal distribution of SNPs and identification of three hot spot mutation regions, labeled (i) motility (flagellar apparatus), (ii) metabolism and transport (trehalose-specific phosphotransferase system [PTS]), and (iii) quorum-sensing (agr-2 operon).