FIG 2.

Competition binding and neutralization by human mAbs targeting YFV E protein. (A) Octet biolayer interferometry competition binding of human mAbs against YFV E. Antibodies listed from top to bottom were associated with immobilized YFV E protein, with antibodies shown from left to right tested for their ability to bind in the presence of the first antibody. Binding is expressed as a percentage of residual binding, with black boxes indicating complete competition, gray boxes indicating partial competition, and white boxes indicating no competition. Antibodies were clustered based on their competition profiles and labeled A to F. (B to D) Neutralization of diverse YFV strains assessed by a focus reduction neutralization test (FRNT). Values were fit to a nonlinear regression model using Prism software. Three independent experiments were performed in technical triplicate, with data from a single representative experiment shown.