FIG 1.
The paa operon is upregulated under TMP/SMX treatment. (A) Schematic of phenylalanine and PAA catabolism, where mao encodes the monoamine oxygenase, feaB encodes the phenylacetaldehyde dehydrogenase, and the paa operon (paaABCDEFGHIJKY) encodes enzymes responsible for PAA’s degradation to succinyl- and acetyl-CoA. (B) Relative gene expression of csuA/B major pilin and PAA metabolism genes in WT Ab17978 grown in TMP/SMX compared to Ab17978 grown in LB, as determined by qRT-PCR. Results are depicted as the mean ± standard deviation (SD) from at least 3 biological replicates and are depicted as log2 fold change. Statistical analyses were performed on ΔCT values using analysis of variance (ANOVA) and Tukey’s multiple-comparison test (*, P < 0.05; **, P < 0.01). (C) Proposed model of PAA regulation in A. baumannii. Created with BioRender.com.