FIG 5.

PAA interrupts the antibiotic response of Ab17978. (A) Csu Western blot of Ab17978 grown in LB with kanamycin (1.5 μg/mL) or gentamicin (1.75 μg/mL), with and without 0.5 mM PAA. (B) Csu expression of WT Ab17978 and ΔpaaB grown in LB, TMP/SMX, kanamycin, or gentamicin. (C to E) Representative growth curves of the WT and ΔpaaB strains in LB, kanamycin (2.5 μg/mL), or gentamicin (2 μg/mL). Cultures were normalized to 0.01 and grown for 16 h with shaking at 37°C. Asterisks indicate significance based on multiple unpaired t tests at each time point. Significance of antibiotic growth curves was achieved starting at the 6th time point as determined by Student's t test (P < 0.01). (F) Csu expression of WT Ab17978 and ΔpaaB strains grown in different antibiotics. CB, carbenicillin (64 μg/mL); CIP, ciprofloxacin (0.125 μg/mL); COL, colistin (0.5 μg/mL); ERY, erythromycin (4 μg/mL); IMI, imipenem (0.125 μg/mL); TET, tetracycline (0.125 μg/mL). All results are representative of at least 3 independent experiments.