FIG 3.

Genome-wide interactions of A. tumefaciens replicons. Normalized Hi-C contact maps displaying contact frequencies for pairs of 10-kb bins across the genome of A. tumefaciens in indicated strains. (A) Wild-type C58 (AtWX063) (11); (B) ΔrepB^Ch2 (AtWX089) (11); (C) ΔpodJ (AtWX283); (D) ΔpopZ (AtWX110); (E) ΔpopZ ΔpodJ (AtWX121); (F) Δgpr (AtWX286). x axis and y axis indicate genome positions. To better visualize contacts in the origin region of Ch1, the reference genome of Ch1 is rearranged with the origin (ori1) at the center and the two replication arms on either side. Ch1, Ch2, pAt, and pTi are indicated by green, red, purple, and black bars, respectively. In panel A, the positions of the four origins are indicated on the x axis. L1, R1, L2, and R2 indicate the left arm of Ch1, right arm of Ch1, left arm of Ch2, and right arm of Ch2, respectively. Interarm interactions on Ch1 and Ch2 are circled in yellow. Interactions between origins are circled in orange. The interactions between the arms of Ch1 and the arms of Ch2 are circled in blue. Quantitative analyses of interactions can be found in Fig. S4.