FIG 3.

dnaK1 and dnaJ3 are required for the production of hormogonium polysaccharide (A) Quantification of cell-associated and soluble HPS based on fluorescent lectin staining and lectin blotting, respectively. Error bars = ±1 SD. *, P < 0.05 as determined by two-tailed Student’s t test between the wild-type and each deletion strain, n = 3. (B) Complementation of the dnaK1 and dnaJ3 deletion strains by exogenous addition of HPS. Depicted are plate motility assays of the deletion strains alone (−HPS) or supplemented with HPS from a cell-free culture medium (+HPS). Images were taken at 48 h post hormogonium induction. (C) Quantification of plate motility assays depicted in (B) based on the area covered by a spreading colony. Error bars = ±1 SD. *, P < 0.05 as determined by two-tailed Student’s t test between each deletion strain in the presence or absence of HPS, n = 3.