FIG 4.

cGAS-STING contributes to S. Typhimurium-induced type I IFN response. (A) ELISA analysis of IFN-β production in the supernatant from C57BL/6 (WT), cGAS^−/− or STING^−/− mouse-derived PMs uninfected (mock) or infected with S. Typhimurium for 6 h at an MOI of 10 (n = 3). (B) and (C) qRT-PCR analysis of gene expression in C57BL/6 (WT), cGAS^−/− or STING^−/− mouse-derived PMs uninfected (mock) or infected with S. Typhimurium for 8 h at an MOI of 10 (n = 3). Data in (B) and (C) were normalized to mock-infected C57BL/6 (WT) PMs, mock-infected cGAS^−/− PMs, and mock-infected STING^−/− PMs, respectively (mock, all set as 1). Actin was used as the housekeeping gene. Error bars represent ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001.