FIG 2.
Sulfane sulfur is involved in the process of AdpA regulating target genes. (A) HSnH was used to treat WT and ΔadpA strains harboring pMS82-actII-4p-egfp. (B) HSnH was used to treat WT and ΔadpA strains harboring pMS82-wblAp-egfp. Data are from three independent repeats and shown as the average ± SD. (C and D) EMSA analysis of the AdpA affinity to PactII-4 promoter DNA (C) and the PwblA promoter DNA (D). All lanes contained 20 nM probe DNA, lanes 2 to 5 contained protein with the indicated concentration, and lanes 3 to 5 contained the HSnH (left) or S8 (right). The black arrow indicates the free DNA probe, and red arrows indicate the PactII-4-AdpA or PwblA-AdpA complex.