FIG 7.
Cys62 residue is critical for AdpA sensing sulfane sulfur. (A) Phenotypes of WT, ΔadpA, and complementary strains (ΔadpA::adpAC62S, ΔadpA::adpAC126S, ΔadpA::adpAC187S, and ΔadpA::adpAC307S) grown on YBP medium. Images were captured from both sides of the plates. (B) EMSA analysis of AdpAC62S binding to PwblA DNA. All lanes contained 16.5 nM probe DNA, lanes 2 to 10 contained AdpA, lanes 5 to 7 contained HSnH, and lanes 8 to 10 contained S8. The black arrow indicates the free DNA probe, and red arrows indicate the PwblA-AdpAC62S complex.