FIG 6.
An allele specific inhibitor of LolA causes OM permeability and activation of the Cpx stress response. (Left) OM permeability to vancomycin and novobiocin was assessed in a strain carrying lolA(V24C) upon treatment with increasing concentrations of 2-[(methylsulfonyl)thio]-ethanesulfonic acid (MTSES). Data are from three independent experiments. Averaged density (A600nm) values of antibiotic-treated cultures relative to mock-treated control (set as 1.0) are shown. (Right) Growth (A600nm) of strains carrying lolA(V24C) and a Cpx reporter plasmid (PcpxP-gfp) was measured. Strains either had native nlpE (blue) or were ΔnlpE::spec (orange). In early log phase (arrow), strains were treated with 0.5 mM MTSES (dotted) or vehicle control (1% DMSO) (solid). Fluorescence was measured and normalized to A600nm to calculate fluorescence per cell. Reporter values were normalized to a DMSO-treated control. Data are average ± SD; n = 3.