FIG 4.
ADO shortens MDK99.99 duration. (A, B, D, and F to H) Survival of E. coli after downshift from exponential-phase LB broth to PBS followed by 0 to 24 h treatment to establish MDK values. Samples were treated with 5 μg/mL GEN or 500 ng/mL CIP. (C and E) Figures are data from MDK curves presented in panels B and D, respectively. Control strain survival values after 6 h of treatment were normalized to the mutant strain. For all experiments, ADO did not cause significant changes in growth or survival compared to VEH. Data are the mean of three biological replicates ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001, as assessed by one-way ANOVA with Fisher’s LSD multiple comparison at each time point or Student's t test. Dotted line indicates MDK99.99 threshold. Asterisk denotes significant difference between treatment groups within a strain unless indicated by a bracket for between-strain comparisons. ADO, adenosine; ND, no CFU detected; VEH, vehicle control.