TABLE 3.
Top 50 enriched sequences and PiTou scores of DV2 directed evolution
| Name | Sequence (5′–3′) | PiTou score |
|---|---|---|
| DENV2-C1 | TGRAQRYKRS | 7.33832 |
| DENV2-C2 | TGAARRSKRS | 14.843 |
| DENV2-C3 | TGAAVRSKRS | 12.3488 |
| DENV2-C4 | TGAMRRTKRS | 11.6874 |
| DENV2-C5 | TGRIQRLKRS | 3.4267 |
| DENV2-C6 | TGNSGRHKRS | 11.4735 |
| DENV2-C7 | TGFSTRNKRS | 10.0498 |
| DENV2-C8 | TGAANRVKRS | 11.1661 |
| DENV2-C9 | TGSVQRIKRS | 8.36797 |
| DENV2-C10 | TGVSRRSKRS | 15.3795 |
| DENV2-C11 | TGSTRRDKRS | 7.72902 |
| DENV2-C12 | TGTKGRVKRS | 12.3958 |
| DENV2-C13 | TGTTHRHKRS | 11.0362 |
| DENV2-C14 | TGLPVRSKRS | 12.6207 |
| DENV2-C15 | TGSRTRSKRS | 13.0648 |
| DENV2-C16 | TGSTRRHKRS | 13.4014 |
| DENV2-C17 | TGHVSRSKRS | 12.2249 |
| DENV2-C18 | TGTRNRKKRS | 13.8726 |
| DENV2-C19 | TGFTNRVKRS | 11.247 |
| DENV2-C20 | TGSNSRSKRS | 12.156 |
| DENV2-C21 | TGASSRHKRS | 12.598 |
| DENV2-C22 | TGQVHRSKRS | 11.0663 |
| DENV2-C23 | TGTAKRSKRS | 13.7489 |
| DENV2-C24 | TGNLRRTKRS | 14.5679 |
| DENV2-C25 | TGFSSRSKRS | 14.164 |
| DENV2-C26 | TGGKVRNKRS | 10.82 |
| DENV2-C27 | TGGHVRHKRS | 10.4826 |
| DENV2-C28 | TGSAQRSKRS | 11.1173 |
| DENV2-C29 | TGEKKRAKRS | 11.8098 |
| DENV2-C30 | TGITTRSKRS | 11.6576 |
| DENV2-C31 | TGAHKREKRS | 10.4483 |
| DENV2-C32 | TGGARRQKRS | 14.399 |
| DENV2-C33 | TGLTRRSKRS | 14.9674 |
| DENV2-C34 | TGASRRAKRS | 13.0953 |
| DENV2-C35 | TGHMTRAKRS | 6.18586 |
| DENV2-C36 | TGLKVRHKRS | 11.5383 |
| DENV2-C37 | TGGAKRGKRS | 10.9028 |
| DENV2-C38 | TGHASRNKRS | 11.3823 |
| DENV2-C39 | TGGDARRKRS | 9.84567 |
| DENV2-C40 | TGVASRTKRS | 13.846 |
| DENV2-C41 | TGNYPRNKRS | 7.18517 |
| DENV2-C42 | TGERTRSKRS | 13.0648 |
| DENV2-C43 | TGRRMRSKRS | 11.7847 |
| DENV2-C44 | TGERKRAKRS | 12.2477 |
| DENV2-C45 | TGMNKRSKRS | 12.312 |
| DENV2-C46 | TGHPSRGKRS | 11.0941 |
| DENV2-C47 | TGVRARTKRS | 13.9117 |
| DENV2-C48 | TGRSLRSKRS | 12.3732 |
| DENV2-C49 | TGMPRRSKRS | 15.082 |
| DENV2-C50 | TGRAVRHKRS | 10.7828 |
| DENV2-V1 | TGAGRRSKRS | 14.843 |
| DENV2-V2 | TGGNRRSKRS | 13.4061 |
| DENV2-V3 | TGSKMRSKRS | 11.3468 |
| DENV2-V4 | TGSRNRLKRS | 12.4246 |
| DENV2-V5 | TGMAKRSKRS | 13.7489 |
| DENV2-V6 | TGTAKRSKRS | 13.7489 |
| DENV2-V7 | TGYRQRSKRS | 12.3466 |
| DENV2-V8 | TGLSRRSKRS | 15.3795 |
| DENV2-V9 | TGGFRRSKRS | 11.508 |
| DENV2-V10 | TGRQARSKRS | 11.0661 |
| DENV2-V11 | TGKMRREKRS | 8.66191 |
| DENV2-V12 | TGSNKRHKRS | 10.7461 |
| DENV2-V13 | TGRTGRTKRS | 12.8044 |
| DENV2-V14 | TGERARVKRS | 12.8337 |
| DENV2-V15 | TGRYKRDKRS | 4.10841 |
| DENV2-V16 | TGAGRSSKRS | −4.9918 |
| DENV2-V17 | TGAWRRSKRS | −5.18386 |
| DENV2-V18 | TGAGRRRKRS | 15.0224 |
| DENV2-V19 | TGGKSRVKRS | 13.5558 |
| DENV2-V20 | TGRPVRSKRS | 12.6207 |
| DENV2-V21 | TGHSRREKRS | 12.5334 |
| DENV2-V22 | TGWGKRSKRS | 13.7489 |
| DENV2-V23 | TGTGRRMKRS | 12.5588 |
| DENV2-V24 | TGAGRRIKRS | 13.5353 |
| DENV2-V25 | TGRSKRSKRS | 14.2854 |
| DENV2-V26 | TGSVRRVKRS | 12.5076 |
| DENV2-V27 | TGASHRSKRS | 13.016 |
| DENV2-V28 | TGMSKRTKRS | 14.4649 |
| DENV2-V29 | TGAGRRNKRS | 12.5588 |
| DENV2-V30 | TGPGRRSKRS | 14.843 |
| DENV2-V31 | TGFKHRVKRS | 12.2057 |
| DENV2-V32 | TGGRHRNKRS | 11.2579 |
| DENV2-V33 | TGAGSRSKRS | 13.6629 |
| DENV2-V34 | TGAGLRSKRS | 11.8703 |
| DENV2-V35 | TGAARRSKRS | 14.843 |
| DENV2-V36 | TGAGRRTKRS | 15.0224 |
| DENV2-V37 | TGAERRSKRS | 11.508 |
| DENV2-V38 | TGSKLRSKRS | 12.6269 |
| DENV2-V39 | TGAVRRSKRS | 13.4061 |
| DENV2-V40 | TGTGRRSKRS | 14.843 |
| DENV2-V41 | TGARRRSKRS | 15.626 |
| DENV2-V42 | TGATKRSKRS | 13.8734 |
| DENV2-V43 | TGDGRRSKRS | 14.843 |
| DENV2-V44 | TGSKIRTKRS | 13.2837 |
| DENV2-V45 | TGAGCRSKRS | −5.14866 |
| DENV2-V46 | TGSGRRSKRS | 14.843 |
| DENV2-V47 | TGGTRRSKRS | 14.9674 |
| DENV2-V48 | TGVGRRSKRS | 14.843 |
| DENV2-V49 | TGISKRGKRS | 11.1775 |
| DENV2-V50 | TGSRNRFKRS | 11.0389 |