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Ferric citrate uptake is an in vivo fitness factor. WT HM7 and the ΔfecA mutant were combined in a 1:1 ratio and transurethrally inoculated into CBA/J mice. Competitive indices were calculated 48 h postinfection. Symbols are individual animals; bars are the median. Significance was determined with Wilcoxon’s signed-rank test: *, P < 0.05; **, P < 0.005.
