TABLE 3.
List of strains and plasmids used in this study
| Strain or plasmid | Genotype/description | Reference or source |
|---|---|---|
| Strains | ||
| HM7 | WT cystitis-causing UPEC strain isolated from healthy young woman in 2012 | |
| ΔentB mutant | HM7 entB::kan Kanr | This study |
| ΔfecA mutant | HM7 fecA::cam Camr | This study |
| ΔfecA/ΔentB mutant | HM7 fecA::cam entB::kan Camr Kanr | This study |
| Plasmids | ||
| pGEN eV | Low-copy-no., promoterless plasmid, Specr | This study |
| pBAD eV | pBAD-Myc/His A, low-copy-no., arabinose-inducible plasmid, Ampr | Thermo Fisher |
| pGEN entB | entB with native promoter, cloned from HM7 via Gibson assembly, Specr | This study |
| pBAD fecABCDE | fec operon (fecABCDE) cloned from HM7 inserted into MCS via Gibson assembly, Ampr | This study |
| pGEX-4T-3 LCN | Human lipocalin-2 glutathione S-transferase (GST) fusion protein, Ampr | |