TABLE 4.
Primers used in this study
| Gene or plasmid | Sequence ofa: |
|
|---|---|---|
| Forward primer | Reverse primer | |
| entB b | ATTCCAAAATTACAGGCTTACGCACTGCCGGAGTCGTGTAGGCTGGAGCTGCTTC | CACCTCGCGGGAGAGTAGCTTCCACCAGGCGTCGAATGGGAATTAGCCATGGTCC |
| fecA b | GATGATGGGGAAGGTATGACGCCGTTACGCGTTTTTCGTAAAACAACACCGTGTAGGCTGGAGCTGCTTC | CCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCATGGGAATTAGCCATGGTCC |
| PnativeentBc | CGGTACCAAGCTTCATATGCACAAATCAGCTTCCTGTTATTAATAAG | GAATAGCCATATCATCCTCCACAAAATG |
| entB c | GGAGGATGATATGGCTATTCCAAAATTACAGG | TTCCTGCAGGGCATGCCCCGTTATTTCACCTCGCGGGAG |
| fecABCDE c | GAGATCTGCAGCTGGTACCAATGACGCCGTTACGCGTTTTTCG | CCAAGCTTCGAATTCCCATACCTCATTAGGCACATCGGCCTGCS |
| pGENc | CGGGGCATGCCCTGCAGG | GCATATGAAGCTTGGTACCGGGATCCGC |
| pBADc | TATGGGAATTCGAAGCTTGGGCCCG | TGGTACCAGCTGCAGATCTCGAGC |
| fecA d | CGGAAGGGCCGATCATAAA | TACCTGGAGCAAGGCAAAC |
| entF d | TTCCAGAAACCACGCTGAG | CCCGATAGCTGAACTGGTAAC |
| gapA d | CGACCTGTTAGACGCTGATTAC | CGATCAGATGACCGTCTTTCAC |
a
Primers are listed in the orientation 5′ to 3′. Underlined sequences for mutant construction indicate regions homologous to the gene of interest.
b
Used for lambda red mutagenesis.
c
Used for Gibson assembly.
d
Used for qRT-PCR.