FIG 1.
RDT is involved in the generation of galE mRNA. (A) The gal operon and the mRNA species generated from the operon. Numbers denote the last nucleotide position (from the P1 promoter transcription initiation site, +1) of the preceding gene stop codons in each cistron junction. (B) Northern blot of the galE mRNA. To see if the galE mRNA is generated by RDT, we treated MG1655 cells at an OD600 of 0.6 in LB medium with the indicated concentrations of the Rho inhibitor bicyclomycin (BCM) for 10 min. Total RNA from the cultures was subjected to Northern blotting.