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. 2022 Jun 13;13(3):e01287-22. doi: 10.1128/mbio.01287-22

FIG 2.

FIG 2

A hairpin structure, E-hairpin, at the 3′ end of the galE mRNA. Numbers indicate the nucleotide position from the P1 promoter transcription initiation site. (A) 3′ RACE assay of the galE mRNA, showing that the 3′ end formed at positions 1,166 to 1,172 in the WT (lane 1) but not in the EHMM1 mutant (lane 2). The E-hairpin-restored mutant formed slightly more 3′ ends than the WT (lane 3). (B) The location and nucleotide sequence of the 3′ end of the galE mRNA in the WT gal operon. The galE gene stop codon is red. The galT gene initiator codon and SD sequence are blue and underlined, respectively. A putative hairpin structure, the E-hairpin, is drawn based on its base complementarity at positions 1,142 to 1,161. (C and D) Nucleotide changes (red) in the EHMM1 mutant (C) and the E-hairpin-restored mutant (D). (E) 3′ RACE assay of galE mRNA in the DH1200 mutant that has an additional E-hairpin at 1,200 (lane 2).