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A failure of galT translation initiation causes RDT at position 1,183. (A) Nucleotide sequence of gal mutants that harbor single (galT-SD1), double (galT-SD2), or triple (galT-SD3) nucleotide deletions between the SD sequence and the galT initiator codon. The galE stop codon is red. The galT initiation codon is blue. The galT SD sequence is underlined. (B) 3′ RACE assay of the SD–AUG mutants in the DH1200 background. (C) Northern blot of galE and galETKM mRNAs in the SD–AUG mutants.
