TABLE 2.
List of primers used in this study
| Reference no. | Description | Sequence (5′–3′) |
|---|---|---|
| STL261 | embR-AES 5′ flank forward harboring AlwNI and SnaBI sites | CACCTTTTCAGAAACTGTACGTACACGCCGTGATCGTCAGCATC |
| STL262 | embR-AES 5′ flank reverse harboring AlwNI site | TTTTTTTTCAGTTCCTGTACGCAGATTAGACACGTAGG |
| STL263 | embR-AES 3′ flank forward harboring AlwNI site | CACCTTTTCAGAGACTGTGCATGTGCAGCACGAGCGAA |
| STL264 | embR-AES 3′ flank forward harboring AlwNI and SnaBI sites | TTTTTTTTCAGCTTCTGTACGTAAAGTCGACCAGATAGGCAAAG |
| STL181 | Hyg end sequence forward for embR mutant screening | CGATCCGGAGGAACTGGCGCA |
| VKN415 | pknH end reverse for embR mutant screening harboring NotI site | AGCTGCGGCCGCTCATTCCTTGTTGACTTTGTC |
| STL53 | pknH middle reverse for embR mutant screening harboring HindIII site | AGCTAAGCTTCTATGCGGGTGGCTGCCGAGGAGC |
| VKN277 | embR middle forward for embR mutant screening harboring DraIII site | TTTTTTTTCACAAAGTGGTCAGCAACCGCTGGATGCC |
| VK98 | embR forward harboring NdeI site | CACCCATATGGCTGGTAGCGCGACAGTG |
| VK99 | embR reverse harboring HindIII site | AAGCTTGATCTACGTGCCGCCATGCGT |
| BSL31 | embR promoter forward (500 bp upstream) harboring ScaI site | CACCAGTACTAAAACGGCATTGTTCACCTC |
| BSL32 | embR reverse without stop codon harboring HindIII site | AGCTAAGCTTCGTGCCGCCATGCGTCCC |
| STL265 | embR forward for pDuet harboring HindIII site | CACCTAAAGCTTATGGCTGGTAGCGCGACAG |
| STL266 | embR reverse for pDuet harboring HindIII and AfII sites | TTTCTTAAGCTTCTACGTGCCGCCATGCGTC |
| BSL400 | hspX promoter forward harboring ScaI site | CCCAGTACTAAGTCAATTGACGCCAGA |
| BSL401 | hspX promoter reverse harboring NdeI site | CCCCATATGATGCCTCCTAATCGATGG |
| VK900 | 16S rRNA qRT forward primer | ACGAACAACGCGACAAACC |
| VK901 | 16S rRNA qRT reverse primer | CCAGCAGCCGCGGTAA |
| BSL29 | embC forward qRT primer | AATTGTCCAGTCCCCGTTG |
| BSL30 | embC reverse qRT primer | CAAAGCCTGTAGGTTAGACCG |
| STL1169 | embA forward qRT primer | TGGTTCTACGTCGGCAACTA |
| STL1170 | embA reverse qRT primer | GTCTTTGACTTCGGTGTGCC |
| STL1173 | embB forward qRT primer | TATACGGAGAGCAGCCCAAG |
| STL1174 | embB reverse qRT primer | GCCCACATATTCCTGCAGTG |
| BSL375 | hspX forward qRT primer | ACATTATGGTCCGCGATGG |
| BSL376 | hspX reverse qRT primer | ACCGACACAGTAAGAATGCC |
| BSL377 | esxP forward qRT primer | GGCAACACGTTTTATGACGG |
| BSL378 | esxP reverse qRT primer | CATGGTGTCTAGCGAGGTC |
| BSL379 | Rv2633 forward qRT primer | ACATTCACTTCCGCATCGAG |
| BSL380 | Rv2633 reverse qRT primer | GTTCCACTCTTCTTCATACCCG |
| BSL381 | espC forward qRT primer | ATGTGTACTTGACTGCCCAC |
| BSL382 | espC reverse qRT primer | CCTCGCTATATATCTTCGCCG |
| BSL383 | lpqH forward qRT primer | GAGGTGAAGTCCGTTGGG |
| BSL384 | lpqH reverse qRT primer | GGTCCCAGTGATCTTGTAGTG |
| BSL385 | devS forward qRT primer | TCGAAGATCCCAAACCGTTAC |
| BSL386 | devS reverse qRT primer | AGAGTGCCGAACGATTCATC |