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Nicked-DNA binding ability of DndE. (A) Fluorescence polarization assay for the interaction of wild-type DndE with DNA. (B) Fluorescence polarization assay for the interactions of DndE mutants with nicked DNA. (C) Electrostatic surface potential of the DndE monomer viewed in two orientations. dsDNA, double-stranded DNA; dsDNAS, shuffled double-stranded DNA; ssDNA, single-stranded DNA; nDNA, nicked DNA; nDNAS, shuffled nicked DNA.
