TABLE 2.
MS/MS analysis of trypsin-digested CTX-M-15 treated with tazobactam or enmetazobactama
| Sample | Peptide | m/z | Mw (observed) | Mw (calculated) |
|---|---|---|---|---|
| Apo | FAMC(cam)STSK | 466.2036 (2+) | 931.4000 | 931.4012 |
| FAM(ox)C(cam)STSK | 474.2014 (2+) | 947.3956 | 947.3961 | |
| TZB treated | FAMC(cam)STSK | 466.2032 (2+) | 931.3992 | 931.4012 |
| FAMCS($)TSK($) | 428.6873 (2+) | 856.3673 | 856.3692 | |
| FAM(ox)CS($)TSK($) | 436.6848 (2+) | 872.3623 | 872.3641 | |
| FAMC(cam)S($)TSK($)VMAAAAVLK | 589.9706 (3+) | 1,767.8972 | 1,767.8954 | |
| FAM(ox)C(cam)S($)TSK($)VMAAAAVLK | 595.3010 (3+) | 1,783.8883 | 1,783.8903 | |
| FAM(ox)C(cam)S($)TSK($)VM(ox)AAAAVLK | 600.6324 (3+) | 1,799.8828 | 1,799.8853 | |
| ETZ treated | FAMC(cam)STSK | 466.2028 (2+) | 931.3982 | 931.4012 |
| FAMC(cam)STSKVMAAAAVLK | 447.2308 (4+) | 1,785.9014 | 1,785.9060 | |
| FAMCS($)TSK($) | 428.6870 (2+) | 856.3667 | 856.3692 | |
| FAMC(cam)S($)TSK($) | 457.1984 (2+) | 913.3895 | 913.3906 | |
| FAM(ox)C(cam)S($)TSK($) | 465.1953 (2+) | 929.3834 | 929.3855 | |
| FAMC(cam)S($)TSK($)VMAAAAVLK | 589.9693 (3+) | 1,767.8933 | 1,767.8954 | |
| FAM(ox)C(cam)S($)TSK($)VMAAAAVLK | 595.3011 (3+) | 1,783.8887 | 1,783.8903 |
a
Bold text indicates peptides containing a Ser70-Lys73 cross-link. $, cross-link; ox, oxidation; cam, carbamidomethylation.