TABLE 4.
MS/MS analysis of trypsin-digested CTX-M-15E166Q treated with tazobactam or enmetazobactama
| Sample | Peptide | m/z | Mw (observed) | Mw (calculated) |
|---|---|---|---|---|
| Apo | FAMCSTSK | 437.6934 (2+) | 874.3794 | 874.3797 |
| FAM(ox)CSTSK | 445.6902 (2+) | 890.3732 | 890.3747 | |
| FAMC(cam)STSK | 466.2037 (2+) | 931.4001 | 931.4012 | |
| FAM(ox)C(cam)STSK | 474.2013 (2+) | 947.3953 | 947.3961 | |
| TZB treated | FAMCSTSK | 437.6928 (2+) | 874.3783 | 874.3797 |
| FAMC(cam)STSK | 466.2039 (2+) | 931.4005 | 931.4012 | |
| FAMC(cam)STSKVMAAAAVLK | 447.2319 (4+) | 1,785.9059 | 1,785.9060 | |
| FAM(ox)C(cam)STSKVMAAAAVLK | 601.3008 (3+) | 1,801.8878 | 1,801.9009 | |
| FAMCS($)TSK($) | 428.6876 (2+) | 856.3678 | 856.3692 | |
| FAM(ox)CS($)TSK($) | 436.6857 (2+) | 872.3641 | 872.3641 | |
| FAMC(cam)S($)TSK($) | 457.1988 (2+) | 913.3904 | 913.3906 | |
| FAMC(cam)S($)TSK($)VMAAAAVLK | 589.9701 (3+) | 1,767.8957 | 1,767.8954 | |
| FAM(ox)C(cam)S($)TSK($)VMAAAAVLK | 595.3016 (3+) | 1,783.8904 | 1,783.8903 | |
| FAM(ox)C(cam)S($)TSK($)VM(ox)AAAAVLK | 600.6336 (3+) | 1,799.8863 | 1,799.8853 | |
| ETZ treated | FAMCSTSK | 437.6935 (2+) | 874.3797 | 874.3797 |
| FAMC(cam)STSK | 466.2035 (2+) | 931.3998 | 931.4012 | |
| FAMC(cam)STSKVMAAAAVLK | 447.2319 (4+) | 1,785.9059 | 1,785.9060 | |
| FAM(ox)C(cam)STSKVMAAAAVLK | 601.3005 (3+) | 1,801.8871 | 1,801.9009 | |
| FAMCS($)TSK($) | 428.6879 (2+) | 856.3685 | 856.3692 | |
| FAM(ox)CS($)TSK($) | 436.6858 (2+) | 872.3643 | 872.3641 | |
| FAMC(cam)S($)TSK($) | 457.1991 (2+) | 913.3910 | 913.3906 | |
| FAMC(cam)S($)TSK($)VMAAAAVLK | 589.9702 (3+) | 1,767.8959 | 1,767.8954 | |
| FAM(ox)C(cam)S($)TSK($)VMAAAAVLK | 595.3019 (3+) | 1,783.8911 | 1,783.8903 | |
| FAM(ox)C(cam)S($)TSK($)VM(ox)AAAAVLK | 600.6330 (3+) | 1,799.8844 | 1,799.8853 |
a
Bold text indicates peptides containing a Ser70-Lys73 cross-link.