TABLE 1.
Cooccurrence of bee rhabdoviruses 1 and 2 in NGS samples here and in three other studies where BRV-1 was identifieda
| Sample ID | No. of BRV-1 reads |
No. of BRV-2 reads |
BRV-1/BRV-2 ratio |
Country | SRA accession no. |
No. of bees/sample |
NA for library construction |
|---|---|---|---|---|---|---|---|
| SRR3927497 | 9,509 | 765 | 12.4 | Israel | SRR3927497 | 30 | Total RNA |
| DWV | 41,335 | 1,024 | 40.4 | USA | SRR6033679 | 10 | Virus-enriched (encapsulated) DNA + RNA |
| NE_AWD_1442 | 34,115 | 737 | 46.3 | The Netherlands | SRR5109823 | 5 | Total RNA (rRNA depleted) |
| SA_RI_49 | 83,757 | 6,791 | 12.3 | South Africa | SRR5109831 | 5 | Total RNA (rRNA depleted) |
| T_V9 | 104,233 | 181 | 575.9 | Tonga | SRR5109822 | 5 (thoraces only) | Total RNA (rRNA depleted) |
| T_V10 | 38,738 | 12,120 | 3.2 | Tonga | SRR5109821 | 5 (thoraces only) | Total RNA (rRNA depleted) |
| T_T12 | 449,293 | 4,501 | 99.8 | Tonga | SRR5109828 | 5 (thoraces only) | Total RNA (rRNA depleted) |
| T_T23 | 331,169 | 113 | 2,930.7 | Tonga | SRR5109834 | 5 (thoraces only) | Total RNA (rRNA depleted) |
| 4A | 44,492 | 6,741 | 6.6 | Czechia | SRS11094606 | 9 | Virus-enriched (encapsulated) DNA + RNA |
| 6A | 83,517 | 4,475 | 18.7 | Czechia | SRS11094614 | 9 | Virus-enriched (encapsulated) DNA + RNA |
| 7C | 7,029 | 1,072 | 6.6 | Czechia | SRS11094620 | 9 | Virus-enriched (encapsulated) DNA + RNA |
| 9A | 127 | 40 | 3.2 | Czechia | SRS11094624 | 9 | Virus-enriched (encapsulated) DNA + RNA |
| 10A | 16,770 | 1,493 | 11.2 | Czechia | SRS11094627 | 9 | Virus-enriched (encapsulated) DNA + RNA |
a
Sequencing reads were mapped to a hybrid reference sequence consisting of combined BRV-1 and BRV-2 genomes (BRV-1, GenBank accession number MH267692; BRV-2, GenBank accession number KY354234) to prevent the interference of multiple mapped reads. The BRV-1/BRV-2 ratio was calculated from read counts.