FIG 2.

The inhibitory effect of EVs from C. albicans (strain 90028) on yeast-to-hypha conditions is dose dependent and has a long-term effect. (A) C. albicans (90028) yeasts were inoculated into M199 (pH 7) in the presence or absence of C. albicans 90028 EVs in different concentrations (equivalent to 0.1, 0.2, 0.3, 0.6, 1, 1.2, 2.5, and 5 μg sterol/mL). Bar, 30 μm. (B) The inhibitory effect of a single C. albicans EV treatment was tested after 24, 48, 72, and 96 h. Briefly, overnight-treated yeasts were washed with sterile PBS, and aliquots of (2.5 × 10³ cells/well) cells were transferred to fresh M199 medium. Yeasts were incubated under the same conditions without addition of EVs for 24 h, and the differentiation was accompanied microscopically. This step was repeated until the reestablishment of C. albicans filamentation. Bar, 30 μm. Experiments were performed in biological triplicate with consistent results.