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. 2022 Apr 14;13(3):e00301-22. doi: 10.1128/mbio.00301-22

FIG 4.

FIG 4

Thermoresistant molecules carried by C. albicans EVs are associated with yeast-to-hyphae inhibitory effect. (A) C. albicans (90028) yeast cells were inoculated into M199 (pH 7) in the presence or absence of C. albicans 90028 EVs or heat-treated EVs in a concentration equivalent to 5 μg sterol/mL. The effect was visualized after 4, 8, and 24 h. PBS was added alone as a control. Bar, 30 μm. The results shown are representative of three independent experiments. (B) C. albicans (90028) yeasts were inoculated into M199 in the presence or absence of C. albicans EVs, EV-derived protein-rich fraction (PF), or lipid lower (LP) or upper phase (UP) in a concentration equivalent to 5 μg sterol/mL. The effect was visualized after 4, 8, and 24 h. PBS was added alone as a control. Bar, 30 μm.