FIG 8.
C. albicans EVs decrease the capacity of C. albicans to penetrate agar and attenuate virulence in Galleria mellonella. (A) C. albicans (90028) yeasts were treated with PBS (control) or C. albicans 90028 EVs in PBS (5 μg/mL) and then plated onto M199 agar for 7 days. The presence of filamentation (top panels) and agar invasion (bottom panels) was observed only under control conditions. (B) G. mellonella larvae were infected with 105 C. albicans yeast untreated or pretreated with C. albicans 90028 EVs (5 μg/mL for 24 h), and survival was monitored (experiments 1 and 2). PBS indicates uninfected larvae injected with PBS. (C) C. albicans (90028) yeasts were inoculated into RPMI supplemented with FBS (10%) in the presence or absence of C. albicans EVs in a concentration equivalent to 5 μg sterol/mL.