TABLE 1.
Major lipid compounds identified by GC-MS/MS in the lipid LP fractions and EVs of C. albicans and EVs of H. capsulatum and S. cerevisiae
| Peak no. | RT (min) | Compound | Peak relative intensity (%) of fungal preparation/fraction: |
|||||
|---|---|---|---|---|---|---|---|---|
|
C. albicans
|
H. capsulatum
|
S. cerevisiae
|
||||||
| LP | EVs | LP/TLC | EVs/TLC | EVs | EVs | |||
| 1 | 9.42 | 2,3-Dihydro-6-trans-farnesol (6,10-dodecadien-1-ol,3,7,11 trimethyl) | 93 | 63 | 32 | 25 | NDa | ND |
| 2 | 9.47 | 2,6-Cis-farnesol (Z,Z-farnesol) | ND | ND | ND | ND | ND | ND |
| 3 | 9.63 | 2-Cis,6-trans-farnesol (Z,E-farnesol) | ND | 4 | ND | 7 | ND | ND |
| 4 | 9.68 | 2-Trans,6-cis-farnesol (E,Z-farnesol) | ND | ND | ND | ND | ND | ND |
| 5 | 9.81 | 2,6-Trans-farnesol (E,E-farnesol) | 100 | 20 | 5 | 9 | ND | ND |
| 6 | 10.33 | Dodecanoic acid (lauric acid, C12:0) | 14 | 5 | 57 | 67 | 25 | 23 |
| 7 | 11.15 | Tetradecanoic acid (myristic acid, C14:0) | 41 | 4 | 97 | 100 | 6 | 4 |
| 8 | 11.25 | Dodecanoic acid, undecyl ester (C23:0-O2) | ND | ND | 30 | 32 | 6 | 4 |
| 9 | 11.42 | 2,3-Dihydro-6-trans-geraniol (6,10,14-hexadecatrien-1-ol 3,7,11,15 tetramethyl) | 14 | 100 | 8 | 12 | 19 | 9 |
| 10 | 11.63 | Geranylgeraniol (isomer 1)b | ND | 9 | ND | 3 | ND | ND |
| 11 | 11.79 | Geranylgeraniol (isomer 2)b | 10 | 97 | ND | 39 | ND | ND |
| 12 | 11.93 | Ascorbic acid 2,6-dihexadecanoate | 72 | 60 | 100 | 68 | 100 | 100 |
| 13 | 12.11 | (9Z)-hexadec-9-enoic acid (palmitoleic acid, C16:2) | ND | 7 | 3 | 3 | ND | 4 |
| 14 | 12.64 | Squalene | ND | 3 | ND | ND | ND | ND |
| 15 | 12.92 | Octadecanoic acid (stearic acid, C18:0) | 41 | 41 | 62 | 30 | 38 | 36 |
| 16 | 13.12 | Octadecenoic acid (oleic acid, C18:1) | 3 | 30 | 5 | 10 | ND | 14 |
a
ND, not detected (below the method's limit of detection).
b
We were unable to determine the precise structures of these geranylgeraniol geometric isomers through fragmentation spectral library matching.