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. 2022 Apr 27;13(3):e00395-22. doi: 10.1128/mbio.00395-22

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Copyright © 2022 Reslane et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — Proline serves as the substrate for arginine biosynthesis in ahrC and Parc mutants. (A and C) CDM-R growth analysis of JE2 ahrC_C124F and JE2 ahrC_K4N with bursa aurealis transposon insertions in putA (A) and argC (C). (B and D) CDM-R growth analysis of JE2 Parc1, Parc2, and Parc3 with bursa aurealis transposon insertions in putA (B) and argC (D). Data represent results from three technical replicates per strain. Data are represented as means ± SEM (n = 3). (E to H) Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of the JE2 ahrC_C124F mutant (E and F) and the JE2 Parc1 mutant (G and H) grown in CDM-R in the presence of either [¹³C₅]proline or [¹³C₅]glutamate. Data represent mean peak values from three biological replicates.