FIG 5.
AhrC represses argGH and arcB1 transcription. (A) Growth analysis of JE2, JE2 ΔahrC, JE2 ahrCC124F, and JE2 ahrCK4N in CDM-R and JE2 ΔahrC/pNF406 (ahrC complement) in CDM-R. Data represent results from three technical replicates per strain. Data are represented as means ± SEM. (B and C) qRT-PCR assessing the transcripts of argG (B) and arcB1 (C) in JE2 ΔahrC and ahrC mutants in both CDM (light gray) and CDM-R (dark gray). (D) Expression of argD in JE2 ΔahrC and ahrC mutants in CDM (light gray) and CDM-R (dark gray). Results are representative of data from three independent biological replicates performed with two technical replicates in each experiment. Error bars show means ± SEM (n = 3). Statistical significance was assessed using one-way ANOVA followed by Dunnett’s posttest. Asterisks indicate significant differences between WT JE2 and ahrC mutants. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, no significance.