FIG 7.

qRT-PCR assessing arcB1 and argGH transcription in Parc mutants. Quantitative reverse transcription-PCR (qRT-PCR) was performed to assess the expression of arcB1 (A and B) and argG (C and D) in JE2 Parc mutants in both CDM and CDM-R. Data are representative of results from three independent biological replicates performed with two technical replicates in each experiment. Error bars show means ± SEM (n = 3). Statistical significance was assessed using one-way ANOVA followed by Dunnett’s posttest (A and C) and Tukey’s posttest (B and D). Asterisks indicate significant differences between WT JE2 and Parc mutants (A and C) and between Parc mutants in CDM and CDM-R (B and D). *, P < 0.05; **, P < 0.01; ns, no significance.